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Innovating Science AP Biology 9 Biotechnology Restriction Enzyme Analysis
Item #:
2134306
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In this lab students will understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis.
About This Item
Description
Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample while determining the approximate size of the DNA fragments in unknown samples. Meets AP Science Practices 3 and 6, and Big Idea 3. Materials provided for eight lab groups.Features
Materials Needed but not Supplied:
- Agarose electrophoresis chamber Microwave or hot water bath
- Electrophoresis power supply Distilled or deionized water
- Micropipettes w/micropipette tips, 10ul
- Gel staining trays or similar (large enough container to accommodate flat agarose gels)
Includes:
- 200mL Prepared Agarose, 0.8%
- 500mL TBE buffer, 5X
- 60mL DNA Stain, 20X
- DNA Samples, 100ul each
- DNA Marker (Lambda DNA HindIII digest)
- Sample #1 (Lambda DNA)
- Sample #2 (Lambda DNA BstEII digest)
- Sample #3 (Lambda DNA EcoRI digest)
- Sample #4 (Lambda DNA BstEII digest)
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parcel
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Specifications
Allergens:
Contains No Allergens
Maximum Age:
18+
Maximum Grade Level:
HS+
No Choking Hazard